5 Significant ISS Life Science Projects of 2017

Wow. 2017 was a busy year on the ISS! 4 Dragons, 2 Cygnus (Cygni?) and 3 Progress spacecraft delivered ~140 new experiments to 12 bustling crew members living on the orbiting outpost during the year. In other words, of all the payloads ever delivered to the ISS, ~11% were delivered in just this year.

These are in no special order, just off the top of my head. My selection criteria was: projects I think improved ISS micro-g research capabilities, created new opportunities to the micro-g community or had high chance for meaningful results.

Which ISS payloads do you think were significant in 2017?


First Chinese Payload on The ISS

Experiment Name:
NanoRacks-BIT-1 (NanoRacks-Beijing Institute of Technology-1: DNA Mismatch during a PCR Reaction Exposed to the Space Environment)

Principal Investigator:
Yulin Deng, Beijing Institute of Technology (BIT), Beijing, China

NanoRacks, LLC, Webster, TX, United States
Beijing Institute of Technology, Beijing, China

The science behind this project was like most experiments going to the ISS: basic, but has potential for important results. So why was it the first one I thought of?

Because  one of the barriers to micro-g research is access. This project introduced a new route for researchers that wasn’t there before.

Yes, Chinese researchers have access through its own space program, but their launches are infrequent and research opportunities even more so. We have ~10 launches per year traveling to a multi-billion dollar micro-g research platform orbiting the planet with the word “international” explicitly in its name. Why can’t China participate?

The BIT module on the ISS before installation onto the USB hub NanoRacks Frame-1. This module was 4U (40 cm x 10 cm x 10 cm).

Worried that China would take advantage of technology transfer (Google “intelsat 708 china ITAR”), Virginia Representative Frank Wolf inserted a clause into the 2011 U.S. budget stating,

“(Sec. 539) Prohibits the use of any NASA or OSTP (Office of Science and Technology Policy) funds to participate in any way in any program with China or any Chinese-owned company, unless specifically authorized by law.”

Since NanoRacks is a separate commercial entity, this project was therefore legal. After getting acceptance from all ISS partners, U.S. Congress, and ensuring no data transfer was possible, the project was then allowed to fly to the ISS.

I’m positive you will see more collaborations of commercial companies and non-traditional ISS researchers on the ISS. Let the democratization of space begin!


East Your Space Veggies!

Experiment Name:

Principal Investigator(s):
Gioia D. Massa, Kennedy Space Center, Kennedy Space Center, FL, United States
Howard G. Levine, Ph.D., NASA Kennedy Space Center, Cape Canaveral, FL, United States

NASA Kennedy Space Center, Cape Canaveral, FL, United States

A continuation of the successful Vegetable Production System (a.k.a. Veggie) installed in 2014, this year the crew grew more variety than ever: Waldmann’s green lettuce, mizuna mustard  and Outredgeous Red Romaine lettuce. The crew was also allowed to eat the lettuce they grew.

NASA astronaut Peggy Whitson tends to the space farm on the ISS. Photo: NASA

I really have nothing else to say about it. As a home vegetable gardener this project always thrills me and with every new vegetable they grow, we get closer to a sustainable food source in space. I’m eager to see them grow tomatoes!


The ISS National Lab Becomes More Lab-like

Experiment Name:
Genes in Space-3 (Genes in Space-3)

Principal Investigator:
Sarah Wallace, Ph.D., NASA JSC, Houston, TX, United States

Boeing, Huntsville, AL, United States
NASA Johnson Space Center, Houston, TX, United States

Like peanut butter and chocolate, the marriage of these two research devices on the ISS was a perfect match.

In what was by far the most exciting experiment to me in 2017, astronaut Peggy Whitson picked microbial colonies from an agar plate, extracted DNA from them, amplified the DNA in the samples using the miniPCR and then ran those PCR products through the MinION DNA sequencer.

NASA astronaut Peggy Whitson working in the Microgravity Science Glovebox (MSG) picking colonies, then placing them into PCR tubes for sample prep. The initial work was done in the MSG due to NASA safety regulations. Unknown, isolated colonies are typically labeled as BSL 2 by default. Animated GIF: NASA

It’s important to note that colonies on the plate were grown from swab samples collected around the ISS, so the Johnson Space Center researchers that designed the experiment didn’t know what DNA sequences they were going to see. This was not a tech demo like last year, but an actual “we don’t know what we are going to find” experiment!

NASA Astronaut Peggy Whitson at the Maintenance Work Area (MWA) on the ISS. The miniPCR is seen just in front of her connected to the tablet. Note Skittles on the wall. Photo: NASA

Both PCR thermocyclers and DNA sequencers are common Earth life science lab equipment and performing the assay Peggy completed is an almost trivial process on the ground, but had never been done collectively like this on the ISS.

I really hope to continue seeing the cross utilization of fundamental life science hardware like this on the ISS. And, since there have now been several demonstrations that pipetting small volumes of liquids in micro-g is not the nightmare once thought, I guarantee you there will be more experiments like this in the future. The ISS National Lab will finally start behaving like an actual lab.




Bacterial Resistance is Futile!

Experiment Name:
EcAMSat (E. coli AntiMicrobial Satellite)

Principal Investigator:
A.C. Matin, Ph.D., Stanford University, Stanford, CA, United States

NASA Ames Research Center, Moffett Field, CA, United States

This project got little fan-fair, but I thought it was important. The first to use the new “doublewide” cubesat format launched from the ISS, EcAMSat set out to test bacterial antibiotic resistance in microgravity.

As several lines of evidence now show, the trend of bacterial populations requiring higher concentrations of antibiotics than on earth poses a dangerous future for space travel.

Deployment of EcAMSat from the ISS by the NanoRacks Cubesat Deployer (NRCSD). Animated GIF from NASA

The shoe box (if you wear 12 EEE) sized  autonomous satellite housed fluid reservoirs, a 48 well sample holder, pumps, LED temperature control, detectors and solar panels.

Following a growth period for the E. coli, the antibiotic gentamicin was introduced to the samples, then a blue dye was injected into the wells to measure the viability of the E. coli. Living E. coli metabolizes the dye and turns it pink.

Guts of EcAMSat. Bags contain media, wash, antibiotic and dye solutions.

The PI also flew E. coli with a rpoS gene mutation. The rpoS gene produces a protein that helps defend against the bacteria against gentamicin. A detector measures the amount of bacteria that are alive (pink) and dead (blue) over time, therefore measuring the efficacy of the antibiotic.

Details of the EcAMSat bacterial detection system. Each of the 48 wells was about 1 uL.

Why a cubesat instead of staying on the ISS? Other than setting up the deployers for launch, there is no crew handling required. The ISS can be a relatively noisy micro-g environment, with fans, crew exercising, ship docking bumps, etc. that causes unwanted vibrations or jostling of your experiment. A cubesat is very quiescent, allowing for a more definitive micro-g experiment.

Also, this project builds upon a previous NASA Ames cubesat called PharmaSat that launched in 2009. I believe Ames and other PI’s are looking to launch cubesats on longer duration and deep space biological space experiments (i.e. BioSentinel ) and will use the experience of this project as mission assurance for future designs.

Experiments like these provide important steps in understanding how and what we need to adapt to life in space for long duration. It would suck to overcome all the significant technological hurdles of long duration spaceflight just to be taken out by lowly microbes.


“Payload hardware and experimental protocol development to enable future testing of the effect of space microgravity on the resistance to gentamicin of uropathogenic Escherichia coli and its σs-deficient mutant”  Life Sciences in Space Research, Volume 15, 2017, Pages 1-10, ISSN 2214-5524


Oh, to Be a Fly on the ISS Wall

Experiment Name:
Fruit Fly Lab -02 (FFL-02) (The effects of microgravity on cardiac function, structure and gene expression using the Drosophila model)

Principal Investigator:
Rolf Bodmer, Ph.D., Sanford Burnham Medical Research Instititue, La Jolla, CA, United States

Karen Ocorr, Sanford Burnham Research Institute, La Jolla, CA, United States
Sharmila Bhattacharya, Ph.D., NASA Ames Research Center, Moffett Field, CA, United States

NASA Ames Research Center, Moffett Field, CA, United States


Experiment Name:
Fruit Fly Lab-03 (FFL-03) (Does Spaceflight Alter the Virulence of a Natural Parasite of Drosophila)

Principal Investigator:
Subha Govind, The Graduate Center of CUNY and Biology Department The City College of New York, New York, NY, United States

Sharmila Bhattacharya, Ph.D., NASA Ames Research Center, Moffett Field, CA, United States

NASA Ames Research Center, Moffett Field, CA, United States

I know these are two experiments, but they both use the same hardware and I found the combination of a great model organism with low tech flight hardware a noteworthy achievement in micro-g science.

First flown during the Heart Effect Analysis Research Team conducting FLy Investigations and Experiments in Spaceflight (HEART FLIES) project in 2014, this is the third flight of their modular fly hotel system. It is a small 1.5U (15 cm x 10 cm x 10 cm), passive module and only requires specific launch and return orientations. FFL-02 used temperature control, but it’s modular design that fits nicely into BioServe’s SABL incubator.

Sharmila Bhattacharya (standing) and Curran Reddy hold an early version of the Fruit Fly module and tubes. Photo: NASA.

Why is this important? The well-studied, model organism Drosophila melanogaster is a great animal to investigate the effects of microgravity on living things. They have a short life cycle and genetically they are ~60% similar to humans with about 75% of human disease genes having a match to a fly gene.

Each  Fruit Fly Inn contains small, 1.25” x 4“ tubes that have a food blob on one end and a cotton plug in the other. A few flies are placed into the tubes before launch, then once in space, they enjoy their deluxe accommodation in the sky by eating and mating. Within a few weeks you have a new generation of flies that developed and lived  their whole life exclusively in space.

Fruit Fly container tubes flown to the ISS. The blue and tan-colored substances are food. The white plugs on top are cotton filters that allow the passage of air. Flies and pupae are visible on the tube walls. Photo: NASA.

Keep in mind that there each module has 15 tubes, so you can send hundreds of flies to the ISS and return thousands using a small volume of space. Add the fact that the modules can be passive and you have a low tech, cost effective, high science impact experiment.

Overall, Sharmila, Karen and their collaborators have studied the effects of microgravity on fruit fly heart formation, the effect of a pathogen on the immune system of Drosophila, and neurobehavioral changes in the flies during spaceflight, all with these simple modules.

My favorite quote from Sharmila is “The access to quality microgravity has changed. I have flown more experiments in the past three years than I did 12 years prior.”

It sure has and I eagerly anticipate to see what 2018 brings.

Dragon. Cygnus. What’s the Difference?

Uber, Lyft. Both are rideshare services. Why do some people use one or the other? It’s typically differences like service availability, pricing variations,  or car/driver personalities.

Dragon, Cygnus. Both are rides to the International Space Station (ISS), so why do some payloads fly on one and not the other? Are they really that different? Does one have a furry pink mustache on the grill and the other doesn’t?

For a researcher flying a microgravity payload to the ISS, it’s good to know the similarities and differences when working with a payload developer. Here are some background, stats, and capabilities that can help clarify which may be the best one for your rideshare to space!


Orbital ATK Cygnus
Cygnus is an expendable cargo spacecraft currently launching to the ISS and was developed by Orbital Sciences ATK headquartered in Dulles, Virginia. Orbital ATK is a commercial company contracted by NASA through its Commercial Orbital Transportation Services (COTS) and Commercial Resupply Services (CRS) agreements to design, build and fly Cygnus. Orbital ATK was recently awarded a portion of the second phase of CRS, called CRS-2, to fly cargo to the ISS at least until 2014.

Nine Cygnus spacecraft have been launched since September 2013, with eight arriving at the ISS and seven delivering science cargo. The initial flight was a demo flight and in October 2014 one vehicle was destroyed when the the booster rocket failed shortly after launch.

Having the capacity to launch 3,500 kg in it’s ~25 m3 cylindrical (some say it looks like a beer keg) pressurized section, Cygnus can haul a lot of mail. While it has mostly been using only its pressurized payload volume for cargo, Cygnus has recently been flying cubesat deployers on the outside.


Grappling the Swan. The ISS robotic arm grabs or “grapples” the spacecraft shortly before attaching it to the ISS. The pressurized portion of Cygnus is the large silver cylinder. Connected below that are the service module and solar arrays. The white box is a NanoRacks cubesat deployer. Photo: NASA.


Cygnus can provide a standard suite of temperature control hardware, typically called “cold stowage,” for your samples or payload on the ride up. Temperatures ranging from -95C to +40C can be accommodated. They can even provide specialty temperatures, if needed.


Winter is Coming to the ISS. When flying your project, you have many options for temperature control. This service is contracted by NASA and available to any payload developer and are suited to fly on Cygnus or Dragon. Click to enlarge. Picture is from NASA Cold Stowage Brochure.


When it comes time to hand over your payload to NASA or your payload developer, two time slots are available: nominal load or late load time. These deadlines are written as “L (for launch) – (minus) (days/hours/weeks/months.)”

For example, the nominal load time for Cygnus is L-8 weeks, meaning you turn over your nominal payload 8 weeks before the scheduled launch date.

A nominal load time typically means your payload doesn’t have any special time critical storage requirements before or during the ride to the ISS. This loading time is commonly used for instruments, tools, crew clothing, or spare ISS parts for example.


Down the Hatch! In this pic, (looking down the long cylinder axis of Cygnus) the loading crew is using a crane to transport heavier payloads through the hatch and into the vehicle. Photo: Orbital ATK.


Late load time is the latest a payload can be delivered before launch for stowage on the spacecraft. Payloads needing this option have specific requirements, typically due to perishable items that have an expiration date or require a temperature controlled environment.

Examples of this may be life science experiments or food items. These are special cases and, because of that, are space limited. For many life science applications, late load is almost a given, but you still must articulate a need for it before receiving this service.

For Cygnus, the late load time is currently at ~L-5 days. The cargo loading process for Cygnus is done while the spacecraft is horizontal and loading is done front to back, either by hand or sometimes with a small crane to carry larger or heavier payloads inside. The cargo crew then attaches your project or clean underwear for the astronauts or whatever to the interior wall of the vehicle.


Interior View of Cygnus During Loading. This view is from Cygnus’ main (and only) hatch. After a long day of loading, these two cargo loading experts are kneeling and praying for a good launch. Photo credit: NASA/Ben Smegelsky.


Next, Cygnus is enclosed in a fairing that surrounds and protects it from aerodynamic, acoustic and thermal forces during launch. This 4 m x 10 m structure is normally jettisoned ~4 minutes after launch.


This Fairing Mating GIF Needs Narration by Sir David Attenborough. The fairing on the right is slowly rolled into position by hand (see the guy underneath!) around Cygnus (center). Cygnus is already mated to its service module (silver thingy), it’s second stage (black tube) and just beyond that, the first stage rocket. Animated GIF from this video.

Lastly, there is what’s called the loiter phase. After launch, there is a time between reaching orbit and catching up with the ISS. So the spacecraft “loiters” during this time. Average loiter time for Cygnus is 3.4 (± 1.2) days over seven missions.

The challenge for researchers is that the late load and loiter times for Cygnus are currently the longest for both vehicles. This can add up quickly. If you have a life science project with a specific shelf life and you handover at L-5 days, plus the 3-4 days loiter time, then another day before the astronauts unload and begin your experiment, it can be 9-10 days before it is started on the ISS.

Breaking Up is Not Hard To Do. Cygnus and its cargo of dirty astronaut underwear and other garbage becomes a beautiful streaking meteor upon re-entry. Photo: NASA

While Cygnus may burn up on re-entry, it is far from being wasteful and can carry a lot of ISS garbage along with it. Orbital ATK has also been working with companies and researchers that want to utilize the vehicle after it leaves the ISS.

The Houston based aerospace company NanoRacks has been deploying cubesats from Cygnus after it leaves the ISS since November of 2016 and NASA researchers have used it for a series of microgravity fire projects called Spacecraft Fire Experiment (SAFFIRE).


Cubsats! Pew! Pew! An external view of Cygnus with its solar array on the left and the NanoRacks deployer on the bottom right as it kicks out a cubesat. Full video here.


Overall, in it’s current configuration, Cygnus can haul a lot of pressurized cargo up to the ISS. It’s lengthy pre-processing flow doesn’t allow for many perishable science items to fly on it, but it has gotten better.

It also seems this spacecraft has plenty of potential uses for researchers and tech demos in the future and I look forward to seeing how it adapts to fit customer’s needs over the coming years.


Space-X Dragon
The Dragon spacecraft is constructed and flown by Space Exploration Technologies Corporation (SpaceX) of Hawthorne, California under the same NASA COTS and CRS/CRS-2 contracts. Like Cygnus, SpaceX is obligated to fly additional cargo flights to the ISS at least until 2024.

Dragon first arrived at the ISS with cargo in May of 2012 and there have been 10 more since, with one failure and loss of vehicle during its ascent in June of 2015.

Dragon has a gumdrop shaped pressurized capsule for internal payloads and a cylindrical external un-pressurized cargo section called a trunk. It can carry a whopping total of 6,000 kg of cargo in combination of internal and external payloads. The usable internal and external volumes are 11 m3 and 14 m3, respectively, for a combined usable payload volume of 25 m3.


Dragon Berthed to the ISS. The gumdrop shaped white part is the pressurized portion, while the trunk is just below with solar arrays extended. Photo: NASA


Science Junk in the Trunk. Underneath and inside the Dragon truck are several external projects ( Defense Department’s STP-H5,  and SAGE 3 instrument, its hexapod attach mechanism flown on CRS-10) mounted to the trunk. Once berthed to the ISS, these payloads will be extracted by the ISS’s robotic arm and then attached to the ISS. Photo: NASA


Dragon is loaded with nominal payloads while it is vertical, then late load is installed while horizontal. Its nominal load time is L-6 weeks.


I Like to Move It, Move It! This view is from the side hatch. Heavier payloads are brought in using a crane, through the main hatch seen above. Another shove and nudge will do it guys. Animated gif from this NASA video


Dragon doesn’t have a fairing like Cygnus, allowing for payloads to be packed relatively soon before it’s rolled out to the pad. Sometimes, when the weather allows, Dragon/Falcon are horizontal at the pad while loading late cargo.

Also in contrast to Cygnus, Dragon has relatively reasonable late load times of L-72h, L-48h and L-28h. The only vehicle that ever came close to that was the Space Shuttle at L-24h and with some extreme cases even closer to launch time.


Dragon and Falcon Basking in the Sun. A scissor lift truck parked next to to the Dragon clean room delivers late load payloads for loading onto Dragon CRS-8 flight in April 2016. This process is typically completed 12-15 hrs before launch. Photo: SpaceX.


Loiter time for Dragon is also, on average, better than Cygnus, at 2.3 (± 0.7) days over 11 flights. Temperature control for your samples/payload is available for all phases of flight.

The other important service Dragon performs is that it’s currently the only cargo vehicle capable of returning a significant about of items back to Earth. Returning up to 3,000 kg of payloads, samples and hardware, Dragon performs a vital service to the ISS microgravity research community.

There is a gotcha with this, though: after splashdown off the southern coast of California, the spacecraft is loaded onto a boat and rides to port. Lately, it has been arriving in port at about 24-30h after splashdown, but it can be up to three days, depending on weather and sea state. For many projects, especially live rodents, this can possibly alter your micro-g results.


Dragon Splashdown. This occurs around 3-4 hours after unberthing from the ISS. Photo: SpaceX.


I’m on a Dragon Boat! After retrieval, the ship motors to a port near Los Angeles to disperse its time critical Dragon treasure. The rest of the payloads will travel to a SpaceX facility in McGregor, TX for unloading there. Photo SpaceX


The good news is, once Dragon arrives in port you have the option of picking up your payload right there or have your payload developer overnight ship it to you. If you don’t need it that quickly, your project can be returned in 4-6 weeks, sometimes sooner. I have handed researchers their payload at the port and had them texting me pictures and data within a couple hours of pick up. I love seeing that.

Dragon has some overlapping capacities as Cygnus, but has some distinct differences as well. The late load time and return services make it attractive to many researchers. The external trunk portion of Dragon is great for hauling projects that need to be mounted on the outside of the ISS. So far, Bigelow Aerospace has made the most use of the trunk with it’s Bigelow Expandable Activity Module (BEAM), taking up the entire trunk volume and weighing in at 1, 413 kg.


One of the most difficult parts of planning for a microgravity research project is when a launch scrubs. This all too common occurrence adds precious time to an already long work flow and is unfortunately one of the many deal breakers for life science payloads.

Some life science projects like seeds or samples that can be frozen indefinitely are well suited for this, but others like cell samples, microbiology, or protein crystal growth just don’t like sitting around for a long duration.

The main distinction between Dragon and Cygnus for scrub scenarios is, as mentioned previously, the Dragon capsule is accessible through the side hatch when the rocket is horizontal. After a launch attempt or two, the rocket is made safe, brought horizontal and sensitive samples are swapped out. 12-15 hours after the scrub the investigator or payload developer gets 15-30 min to swap hardware or samples and they are reloaded onto Dragon.

The fairing around Cygnus lacks an access port, so there is no way to enter the vehicle with it installed. The rocket has to be rolled back to the processing facility and the whole fairing has to be removed.

How long does this take? I don’t know. While there have been delays and scrubs for Cygnus, in eight launch attempts a full rollback and re-load has only ever been done once during Orb-1. And that roll back and de-stow was ollowed by a 23 day delay until it launched.


Which Spacecraft Is Right for Your Research Payload?
When you work with a payload developer, they will help you with this decision process. There is a whole suite of paperwork and matrices that NASA and its contractors use to determine what vehicle is best for you and when there is room on the manifest for it.

So, all of the above was just to say: it really comes down to how delicate your samples are.

Are they stable or can be stabilized for at least 10 days or more? Then Cygnus or Dragon will work for you.

If you absolutely require the latest load time available or need your samples back, then Dragon is the only ship for you.

Here is a nice side by side comparison of the vehicles:


Side-by-Side Graphical Representation of Dragon and Cygnus to Scale. Currently, only the extended versions are used. Red = pressurized volume. Yellow = un-pressurized. Graphic by Craigboy, modified from original creative commons  Wiki pic.

Comparison Table for Cygnus and Dragon.


But Wait, There’s More! New ISS Cargo Transport Option on the Horizon!
One of my biggest gripes with the current capabilities of these cargo ships is that there is currently only one that has reasonable late load times and can return a substantial amount of pressurized cargo back to Earth.

Well, I’m hoping those challenges of the research community will be eased in a new cargo vehicle: Sierra Nevada Corporation’s Dream Chaser Cargo System. Allow me to swoon a bit.

Awarded as part of the second phase of the CRS funding selections in January 2016, Sierra Nevada is obligated to provide six flights to the ISS.

Dream Chaser is a lifting body type spacecraft that looks like a baby shuttle. It will be even be reusable like a shuttle in that it can return from space with a gentle 1.5g flight path and land on a 3, 000 m runway.

With wings that fold up, it will be able to fit into the fairing of several types of rockets. To get around the shortcoming that Cygnus has, the fairing will have an access port so that late load payloads can be added far into the launch preparations, even possibly while on the pad.


Dream Chaser Cargo. Cargo carrying mini-space plane in the front, with a service module and solar arrays in the back. Photo: Sierra Nevada Corporation

Sierra Nevada is advertising Dream Chaser will have the capacity to lift up to 5,500 kg of pressurized and 500 kg un-pressurized cargo to the ISS. It will also be able to return 1,750 kg of payloads from the ISS and since the spacecraft will also use non-toxic chemicals for its systems, so safing the vehicle after landing will allow for very quick recovery of time critical payloads.


Dream Chaser After Landing During an Un-powered Glide Test. It’s so cute! Good pic to give you a sense of its size. Photo: Sierra Nevada Corporation


Dream Chaser Cargo just had its first successful glide test and is on target for its first flight to the ISS in 2020. The value of having a cargo vehicle that can do all of that will be a great asset to the microgravity research community and I simply cannot wait.



More on NASA CRS

Orbital ATK Website

SpaceX Website

Data for cargo mass averages from NASA CRS Media Releases

What’s Up With OA-7?

The third launch of Cygnus using an Atlas V-401 booster commenced successfully on Tuesday April 18th from Cape Canaveral Air Force Station in Florida and berthed nominally to the ISS almost four days later.

OA-7 launching from SLC-41 at Cape Canaveral Air Force Station in Florida. Source: ULA

OA-7 sees the return of refrigeration/freezing (i.e. Polar) stowage, an asset missing since Orb-3, which helps offset the increased late load for those science experiments that can be frozen. Because of the fairing surrounding Cygnus, it has a 10+ day late load capability and hasn’t been used much for perishable science that requires a short duration between handover and berthing/de-stow.

For reference, late load science for this mission was handed over and stowed on Cygnus during the first week of March (when the launch date was March 24th). Late load for biologicals on Dragon can be as late as 28 hours before launch.

Inside the Cygnus OA-7 pressurized cargo section. Note the four Polar freezers in the forefront. Picture: NASA.

Cargo By the Numbers
The science cargo mass (table below) is a little higher than previous missions, likely due to the refrigeration support hardware and the Saffire-III experiment, both which are large and heavy. Crazy to see that the Atlas V 401-Enhanced Cygnus config allows for 64% more total pressurized mass to LEO than the Antares 230-Enhanced Cygnus, yet about the same amount of science mass on both. With an average of 1,513 lb, the science mass carried on Cygnus for OA-7 is comparable to the average amount carried by SpaceX’s Dragon at 1,375 lb.

The Science!

Magnetic 3D Cell Culture for Biological Research in Microgravity (Magnetic 3D Cell Culturing)

Principal Investigator
Glauco Souza, Ph.D., Nano3D Biosciences, Inc., Houston, TX, United States

Payload Developer
BioServe Space Technologies, University of Colorado, Boulder, CO, United States

Culturing cells in 3D has gained significant attention in Earth labs over the past decade. The technique removes cells from the standard two dimensional monolayer methods used since the 1800’s and attempts to create a more natural three dimensional growing environment that facilitates the cell-cell communication and structures that tissues would normally physiologically experience. Drug efficacy can be different between cells grown in 2D and 3D and it has therefore captured notable interest, especially in the biopharma world.

Growing 3D cell cultures typically requires special plates, a bioreactor or gel scaffold, but in 2008 researchers at Rice University and at the University of Texas MD Anderson Cancer Center, both in Houston, Texas, developed a way to levitate cells using magnets, so that they can grow three dimensionally. This magnetic levitation method (MLM) has since been commercialized by those researchers with a company named Nano3D Biosciences (n3D).

Source: n3D Biosciences

This CASIS funded experiment is a technology demonstration/validation of n3D’s technology as a tool for growing and handling cells in microgravity. Lung carcinoma cells are launched frozen, thawed and the crew will inject them into media before incubation. At some point the crew will manipulate them with the n3D magnetic technology and observed with on-board microscopes. The samples will be then be fixed, frozen and returned at a later time.

It’s unclear what the manipulations will be, since MLM is beneficial for 3D cell growth in 1g, yet that’s not necessary in a microgravity environment. It will be interesting to see what they come up with.


Efficacy and Metabolism of Azonafide Antibody-Drug Conjugates (ADCs) in Microgravity (ADCs in Microgravity)

Principal Investigator
Sourav Sinha, Oncolinx LLC, Boston, MA, United States

Payload Developer
BioServe Space Technologies, University of Colorado, Boulder, CO, United States

Oncolinx was founded in 2014 as a spin off company from the technology accelerator Center for Advancing Innovation in Bethesda, Maryland. Partnered with the National Cancer Institute,
Oncolinx has developed and patented antibody-drug conjugate (ADC) azonafides, a class of DNA intercalating anticancer compounds.

Oncolinx has won several grant funding competitions such as 43North Startup Competition, Breast Cancer Startup Challenge, and the MassChallenge, to name a few. They currently have partnership agreements with eighteen universities and pharma companies and are scheduled to start human trials in 2017.

ADC’s are a relatively new type of anticancer drug delivery method, where like a Trojan horse, a drug is attached to an antibody that is designed to target a cancer cell type. The cancer cell readily recognizes and absorbs the ADC where the drug is then released and kills the cell. This strategy removes the nonspecific “kill em all, let the body sort em out” drugs employed in standard chemotherapy. While incredibly promising, there are currently only two ADC’s approved by the FDA.

Illustration of ADC-azonafide activity. Source: Oncolinx

The experiment hopes to shed light on the mechanism of ADC azonafides in cells grown in microgravity. Expectations of how this will be different than Earth 3D cultures isn’t mentioned. This and the previous CASIS funded experiment are related in that they are both using Nano3D Biosciences technology and lung cancer cells.

Like most microgravity cell biology experiments, the protocol is rather simple. Frozen cells and drug are launched to the ISS, where they will be thawed and introduced into media with BioServe’s multi-well BioCell hardware. They’ll be incubated and manipulated with n3D technology, observed microscopically during growth and then fixed, frozen and returned to Earth for further study.


Center for Advancing Innovation

“Assembly of a functional 3D primary cardiac construct using magnetic levitation”


“The Center for Advancing Innovation Spin-Out, Oncolinx, Is the Winner of the Largest Investment Prize for Global Startups”

“A spheroid toxicity assay using magnetic 3D bioprinting and real-time mobile device-based imaging”

Wiki: 3D cell culturing by magnetic levitation

n3D Biosciences

Patent: Azonafide derived tumor and cancer targeting compounds

Wiki: 3D cell culture

Wiki: Antibody-drug conjugate

“Three-dimensional Tissue Culture Based on Magnetic Cell Levitation”


Crystal Growth of Cs2LiYCl6:Ce Scintillators in Microgravity (CLYC-Crystal Growth)

Principal Investigator
Alexei Churilov, Ph.D., Radiation Monitoring Devices, Inc, Watertown, MA, United States

Payload Developer
Radiation Monitoring Devices, Inc., Watertown, MA, United States; NASA Marshall Space Flight Center, Huntsville, AL, United States; Tec-Masters Inc., AL, United States

Detached Melt and Vapor Growth of InI in SUBSA Hardware (Detached Melt and Vapor Growth of InI)

Principal Investigator
Aleksander Ostrogorsky, Sc.D., Illinois Institute of Technology, Chicago, IL, United States

Payload Developer(s)
Illinois Institute of Technology, Chicago, IL, United States; NASA Marshall Space Flight Center, Huntsville, AL, United States; Tec-Masters Inc., AL, United States

In another double project, these experiments are funded by CASIS Materials Science Investigations released in 2014. The Solidification Using a Baffle in Sealed Ampoules (SUBSA) furnace has been brought out of 15 year storage and updated for another flight.

SUBSA’s first flight to the ISS was in 2002 (and by coincidence, when astronaut Peggy Whitson was on board) and while the researchers “grew eight single crystals of indium antimonide (InSb), doped with tellurium (Te) or zinc (Zn)” there were several technical issues reported with SUBSA and the newly arrived Microgravity Science Glovebox that may have reduced the experiment’s main goals.

SUBSA Furnace. Source: Tec-Masters

Churilov and Ostrogorsky are also the same researchers that flew is 2002, but this time they will be testing the crystal growth of two different materials: Cs2LiYCl6:Ce (CLYC) and indium iodide (InI), respectively, both of which are important materials used in radiation detection. They wish to grow quality, less flawed crystals in microgravity as well as gather data for a better understanding of the microgravity crystallization process.

These data will be used to increase the detector material’s performance by optimizing certain processes during their manufacture on Earth. These materials have use in “homeland security and nuclear non-proliferation applications, oil and gas exploration, particle and space physics, non-destructive testing, and scientific instruments.”

Defects found in CLYC crystals grown in 1g

Tec-Masters SUBSA Flyer

CASIS Materials Science Investigations RFP

AIAA Abstract for SUBSA

Space Ref:Technical Issues SUBSA/MSG First Run


Thermal Protection Material Flight Test and Reentry Data Collection (RED-Data2)

Principal Investigator
John Dec, Ph.D., Terminal Velocity Aerospace, LLC, Atlanta, GA, United States

Payload Developer(s)
NASA Johnson Space Center, Houston, TX, United States
NASA Ames Research Center, Moffett Field, CA, United States
Terminal Velocity Aerospace, LLC, Atlanta, GA, United States

Great balls of fire! Bet the researchers never heard that one before. Terminal Velocity Aerospace, LLC hopes this experiment will allow us to better understand how objects behave during orbital re-entry. Awarded two Small business Innovation Research (SBIR) grants since 2014, TVA also hopes to increase options for payload return from the ISS by developing 10small payload return capsules.

Even as relatively common as re-entry is, there is still little actual data about what a spacecraft experiences during its fiery demise. Knowing this could help build better models and design objects be destroyed or saved during re-entry.

Areoshells similar to those that will be used in the RED-Data 2 experiment. Source: TVA

Three soccer ball sized experiments will ride with Cygnus as it breaks up upon re-entry and send location, temperature, pressure and acceleration telemetry to the Iridium network as it happens. The REDs are also covered in thermocouples and several types of thermal protection materials that will test their performance after they leave the charred, smoldering remains of Cygnus.


Terminal Velocity Aerospace, LLC



Advanced Plant Habitat (Plant Habitat)

Facility Manager
Bryan G. Onate, Kennedy Space Center, FL, United States

Payload Developers
NASA Kennedy Space Center, Cape Canaveral, FL, United States
Orbital Technologies Corporation, Madison, WI, United States

Plant growth chambers have come a long way since the little Oasis plant systems used on Salyut 7. In the eventual goal of providing sustainable farming for spaceflight missions, the Advanced Plant Habitat (APH) is a companion to the successful VEGGIE project and it brings some lessons learned as well as new plant growth features to the ISS.

VEGGIE was open to cabin air and this environment can be quite variable and cause unwanted effects to plant growth experiments. Examples of this was increased concentrations of the plant hormone ethylene altering wheat and Arabidopsis growth during the Shuttle-Mir Greenhouse and STS-84 projects, respectively.

Advance Plant Habitat. Source: NASA

The APH has a contained growth chamber with air scrubbers, gas mixture, temperature and humidity controls as well as an active watering system. There are variable wavelength LEDs for different light requirements of an assortment of plant types and light sensors that can measure light from the canopy to the roots.

APH will provide real time telemetry to researchers through a package called Plant Habitat Avionics Real-Time Manager in EXPRESS Rack (PHARMER..ha!) They certainly didn’t spare any expense and I think this will likely be one of the most productive microgravity plant habitats yet.

“Review and analysis of over 40 years of space plant growth systems


Genes in Space-2

Principal Investigator
David Scott Copeland, The Boeing Company, Pasadena, TX, United States

Payload Developer
Boeing, Houston, TX, United States; miniPCR, Cambridge, MA, United States

Genes in Space is a yearly science contest where students submit their microgravity experiment ideas of how they would use the miniPCR thermocycler on the ISS. 2016’s winner, Julian Rubinfien, a student at Stuyvesant High School in New York City, proposed a PCR method of measuring telomere length on the ISS. Be sure to catch the announcement of 2017’s winner in July at the ISS R&D conference in Washington, D.C.

Genes in Space 2016 winner Julian Rubinfien accepting his trophy from NASA astronaut Josh Cassada at the 2016 ISS Research and Development Conference. Source: Genes in Space

As evidence builds that stress is a factor influencing telomere length, it’s not surprising that the stress and radiation of spaceflight may be affecting them as well. Susan Bailey, a researcher on the NASA Twins study, even raised a lot of eyebrows earlier this year when she released preliminary data stating that astronaut Scott Kelly’s telomeres may have even grown longer during the recent one year mission, when compared to his brother.

Since the ISS currently lacks ways to implement some of the more involved methods to measure telomere length, Julian proposed using a simpler Earth lab standard technique called Universal Single Telomere Length Analysis (STELA). While STELA typically requires a bit of optimization work on the front end, it requires minimal hardware to run and a great option to try on the ISS.

This proof of concept experiment is quick and easy: his samples will arrive on the ISS frozen, thawed and run on the miniPCR thermocycler. They’ll then be returned to earth for further analysis. Hopefully the results will lead to a standard test that astronauts can use during long duration spaceflight.

From the Genes in Space website, Julien says,

Preach it, Julien.


Genes In Space


2017 ISS R&D Conference in Washington, D.C.

“Telomere Length: A Review of Methods for Measurement”

“Environmental Stresses Disrupt Telomere Length Homeostasis”

“Reduced telomerase activity in human T lymphocytes exposed to cortisol”

“Astronaut twin study hints at stress of space travel”

“Metabolomic and Genomic Markers of Atherosclerosis as Related to Oxidative Stress, Inflammation, and Vascular Function in Twin Astronauts”

“Telomere Length Measurement – caveats and a critical assessment of the available technologies and tools” 


Genes in Space-3
Biomolecule Sequencer

Principal Investigator
Sarah Wallace, Ph.D., NASA JSC, Houston, TX, United States

Payload Developers
Boeing, Huntsville, AL, United States
NASA Johnson Space Center, Houston, TX, United States

In a natural match of molecular biology hardware, this experiment uses the miniPCR and the MinION DNA sequencer (flown to the ISS last year) as a technique demo for possibly testing microbial samples found in the ISS.

Surface sample from the ISS after incubation. Yum!


Current methods require crew to collect air and surface samples, plate them, place them in a warm spot for five days and then send pics to the JSC lab for analysis. If crew can get samples, prep them with the miniPCR and then run them on the MinION, they could possibly detect what is living there genetically.



Whether this is less work for the crew remains to be seen, but it’s a nice demonstration that researchers can use these two devices together for other types of experiments on the ISS. It could also be used during long duration spaceflight for monitoring crew health or samples while on a Mars mission.


Spacecraft Fire Safety III (Saffire-III)

Principal Investigator
David L. Urban, Ph.D., Glenn Research Center, Cleveland, OH, United States

Payload Developer
NASA Glenn Research Center, Cleveland, OH, United States

Until previously, microgravity combustion experiments on the ISS, such as the Burning and Suppression of Solids (BASS) series, have focused on somewhat small scale test objects of about 10 cm. The Saffire series hopes to provide insight on how fire burns in microgravity on a large scale.

Saffire-III builds upon the two mostly identical experiments flown last year on Cygnus, where meter long pieces of common spacecraft materials such as Nomex and plexiglass are set ablaze. The hardware rides inside Cygnus and isn’t started until long after un-berthing from the ISS.

Engineers/technicians working on Saffire-II. Source: NASA

The fire is recorded visually and with a suite of thermocouples, CO2 and pressure sensors to name a few. The data is then downlinked to the researchers on Earth before Cygnus reenters the atmosphere. It’s really great that Cygnus will be delivering valuable science data all the way up until the end.

Saffire-I  burning material. The green LED light flashes are used to show contrast to observe smoke patterns as the material is burning. Gif Source: NASA

Saffire Mission Site

Burning and Suppression of Solids (BASS)


QB50 CubeSats

The QB50 Project is a constellation of science cubesats to measure phenomena in an often overlooked region of Earth’s lower atmosphere. Run by the Von Karman Institute for Fluid Dynamics and funded by the European Commission, 36 cubesats from 21 countries will be eventually be launched into Earth and Sun Synchronous orbits.

Source: QB50 Site

28 cubesats will be deployed from the ISS using NanoRacks’ Cubesat Deployer where they will collect data in Earth orbit for 4-8 months. The remaining 8 cubesats will be placed in a sun synchronous orbit to be launched from an Indian PSLV rocket in late May.

Source: QB50 Site

These data will collected by three main types of sensors found on the cubesats: ion/neutral mass spectrometer, a flux probe and a multineedle Langmuir probe. I won’t even pretend to know how all of this works, but there is a significant amount of information in the links below.


QB50 Project

Von Karman Institute for Fluid Dynamics



Additional OA-7 Resources
Initial Press Release for OA-7

Cygnus Packed with Experiments to Support Future Exploration

How Many Research Payloads Have Flown to the ISS?

I get asked this question every once in a while and I finally got some free time over Christmas break to try and track an answer down. I quickly found a NASA brochure online titled “International Space Station Utilization Statistics Expedition 0 – 44 December 1998 – September 2015” . Seemed like a good place to start and right on the first page I found this:

Easy peasy. “Total investigations from ISS Expeditions 0-44 is 2,060.” There. Finally, an answer I can give those who ask. But is an “investigation” the same as a payload? They give the definition of investigation in the paragraph above the table, but it’s kind of loose and doesn’t really say something is specifically a physical research payload or not.

NASA also maintains a website with a list of experiments by expedition (and a few other categories) and I thought of comparing the number of payloads on that site to the number above. After some Excel wrangling I came up with the following:


Example of raw data from NASA site. Pink cells indicate duplicate entry. Using these data, the total number of payloads (red circle) is 3, 818 since Expedition 0 in 2000. (Click pic to make larger)


Well, the total of 3,818 from the data on that page is a bit larger than the 2,060 reported in the brochure. Even if you include the three additional expeditions since that publication, it only adds up to 2,609. Also, the values I have don’t match the values they have for “Total Investigations” during each expedition increment.

A quick check for duplicate values in the data (pink in the Excel sheet above) showed that many of the experiments are repeated through expeditions. For example, Made In Space’s 3D printer is listed three times in the data (shown as “3D Printing in Zero-G”) because it ran multiple times during several expeditions. I learned from a NASA friend that they call each payload use an “interaction” and as the data above shows, there can be several interactions with a payload over multiple increments. Still don’t know why the totals don’t match up, though. NASA’s data in their brochure doesn’t even match up with the data shown on their website. Alrighty.

Curiouser and curiouser (or more like: nerdier and nerdier), I edited out the duplicates and came up with what I think is a much more reasonable number.


Example of data from NASA site with duplicates removed. Total (in red circle) shows 1,062 payloads since Expedition 0 in 2000. (Click pic to make larger)


OK, 1,062 payloads ~16 years seems like a more reasonable estimate to me. Plotted as a bar graph,

Data from above Excel sheet plotted as a bar graph. Expedition 0 (September 2000-November 2000) on far left of the horizontal axis to Expedition 49/50 (September 2016-February 2017) on the right.  Note that early expeditions were shorter duration. (Click pic to make larger)

It’s great to see a slight upward trend in new payloads since late 2013-early 2014. Since I was already in the thralls of Excel ecstasy, I was wondering what caused the spikes of new payloads, especially between March 2014-September 2015. I plotted this data along with the number of visiting payload vehicles during the increment.

Plot of number of new payloads during increment (blue bars, values on left vertical axis) with number of visiting vehicles during the increment (orange line, values on right vertical axis). Red arrows show significant events during an increment. (Click pic to make larger)

The peaks correlate nicely to a very busy time on the ISS between March 2014 and September 2015 with 12 vehicles arriving: SpaceX had 4 Dragon visits, Orbital had one Cygnus visit, there was 1 ESA ATV and JAXA HTV visit each, and finally 5 Progress vehicles (see below).  The other peaks seen at October 2007-April 2008, April 2009-October 2009 and March 2011-September 2011 correlate to ATV-1, HTV-1, and STS-134, STS-135 ATV-3, respectively.

This certainly shows the value of Dragon and Cygnus at being able to carry a significant amount of science payloads. Dragon is particularity useful for life sciences, not only because of its ability to return samples, but because researchers can deliver their samples to be loaded in the capsule at ~30 hours before launch.


Overall, it looks like a reasonable number for the amount of research payloads flown to the ISS in 16 years is ~1,000. I hesitate to say the number is exactly 1,062 as calculated, because I may be missing something (maybe NASA has an explanation for the numbers they published) and some of the payloads listed on the NASA site are not really research payloads (such as “Crew Earth Observation” or “Story Time From Space”).

We can also see from these data the significant value Dragon and Cygnus bring to the research community. This value will no doubt increase again hopefully in 2019 when Sierra Nevada flies their Dream Chaser cargo vehicle to the ISS, adding more late sample loading and return capabilities. Now if we could just get more crew up there to work on all of this science…